Bulletin of Pharmaceutical & Medicinal Research2959-91992958-65182022-10-13351410.58398/0005.000014articleMuhammad HamzaHamza AsgharJaveria SaghirAiman RiazZarish SuhailDepartment of Zoology, University of Education, PakistanUniversity of Health Sciences, Lahore, PakistanNortheast Normal University, Changchun, Jilin, ChinaUniversity of Health Sciences, Lahore, PakistanUniversity of Health Sciences, Lahore, PakistanDiabetes mellitus (DM) is a chronic metabolic disorder associated with hepatic dysfunction caused by persistent oxidative stress and hyperglycemia. The establishment of safe plant-based therapies to manage diabetes-related hepatic injury has been an important research area. This experimental study determined the hepatoprotective effect of Cichorium intybus (Kasni) seed extract on alloxan-induced diabetes in mice via histopathological evaluation. Thirty male albino mice were selected and randomly divided into three groups (n = 10 each): the nondiabetic control group, the diabetic untreated group, and the diabetic Kasni-treated group. Type 2 diabetes was induced by the intraperitoneal administration of alloxan monohydrate (150 mg/kg). Aqueous extracts of Kasni seeds (400 mg/kg) were orally administered once a day for 28 days to the mice in the treatment group, and gross liver morphology and histological features were studied for changes via hematoxylin and eosin (H&E) staining. The results of the present study revealed that untreated diabetic mice presented elevated blood glucose levels, enlarged pale livers, and histological features indicating hepatic injury, including hepatocellular vacuolation, sinusoidal congestion, and early pericentral fibrosis. Diabetic mice treated with Kasni presented near-normal hepatic histological features; furthermore, the structure of the central vein was restored, orderly hepatocyte plates formed, and no inflammation, steatosis or fibrosis was observed. The gross morphological features revealed that the liver color and texture were similar to those of the control group. The study concluded that Cichorium intybus has a hepatoprotective effect against diabetes-related liver damage because of the antioxidant and anti-inflammatory properties of the active ingredients in the plant.